The role of DNA profiling in monitoring grain quality

Bacteria growing in petri dishes.
Portrait photo of Dr. Sean Walkowiak

Dr. Sean Walkowiak
Research scientist/program manager
Microbiology and Grain Genomics
sean.walkowiak@grainscanada.gc.ca

The Microbiology and Grain Genomics program conducts research and develops new testing methods in our state-of-the-art DNA profiling lab. We identify the bacteria and fungi found on grains using their DNA and develop DNA-based tests to detect their presence in grain samples. We also use DNA testing to identify crop varieties in grain shipments and currently have a database containing the DNA profiles of more than 800 varieties. The monitoring of microorganisms and grain varieties helps ensure the integrity and marketability of Canadian grain and that the needs of end-users are met.

Studying microbial communities on grain

The bacteria and fungi that make up microbial communities on grains are quite diverse. Some of these microorganisms contribute to the health of plants and soils, some cause harm and others have no known effects. When we find an unfamiliar bacterium or fungus on grain, we determine its DNA makeup through a process called sequencing. The DNA of the microorganism under investigation is compared to that of related species to determine how it differs (Figure 1). We can then target the differences in the DNA using highly specific tests that can accurately distinguish between two DNA sequences. We use the results of these tests to create a DNA profile that is unique for a certain species and can be used to identify it in a sample (Figure 2).

Our high-throughput robotic systems allow us to accurately and efficiently perform DNA tests on thousands of Canadian grain samples every year (Figure 3). The data generated from these tests help us monitor disease-causing organisms in grain more accurately. For example, we have been able to determine the geographical and temporal trends in the fungal species responsible for Fusarium head blight in Canadian wheat (Figure 4). This disease can affect the yield, quality and safety of grain. Understanding how Fusarium species vary across regions and time plays an important role in its management.

The genome data we collect also gives us detailed information about the biology of microorganisms that can be used to assess risks and benefits to crop quality and safety. For example, we can determine which species contain the genes responsible for certain diseases or toxins. Understanding plant pathogens on a genomic level helps producers, breeders, pathologists, and agronomists develop mitigation strategies that target emerging species and strains.

DNA testing of grain varieties

Varieties of grain that are indistinguishable from each other based on physical characteristics or chemical analysis can still differ in their end-use qualities. The DNA-based tests that we develop enable us to accurately identify all varieties present in grain samples, including new varieties of grain that are developed and released by breeders. By sequencing the genomes of new crop varieties (Figure 1), we can detect differences in their DNA and use this information to perform thousands of targeted DNA genotyping tests at the same time (Figure 2). The genome information we gather is also used to identify the genes responsible for traits that are in demand, which helps breeders develop new varieties that meet the changing needs of producers and customers of Canadian grain.

Figure 1  High-throughput robotic DNA sequencing equipment used to gather whole genome information on microorganisms and grain crops.
Laboratory technicians operating DNA sequencing equipment
Figure 2  Cluster plots using example data for three different DNA tests that target a specific area in the DNA. Individuals differ in their DNA sequence and either have “Sequence A” or “Sequence B”.
Three plots of example data for DNA tests with each plot containing 2 clusters.
Figure 3  DNA arrays that allow for thousands of targeted DNA genotyping tests to be performed at once. Pictured below are a Takara SmartChip plate (left) and an OpenArrayTM plate (right).
Two different styles of DNA testing plates with arrays of holes
Figure 4  Relative abundance and diversity of Fusarium species on Canadian wheat by year and province based on DNA testing (n = the number of samples).
Chart showing the relative abundance and diversity of Fusarium species on Canadian wheat.
Graph data
Relative abundance
YearTotal SamplesF. avenaceumF. tricinctumF. acuminatumF. crookwellenseF. culmorumF. equisetiF. graminearumF. langsethiaeF. poaeF. pseudograminearumF. sporotrichioides
201486117622343053473170107617
2015117631417715292018420928201491279
2016945714246683273187122096726
20181247220122103118201240
20195521384533418064630266103
2020505531872572307433908645
Relative abundance
ProvinceTotal SamplesF. avenaceumF. tricinctumF. acuminatumF. crookwellenseF. culmorumF. equisetiF. graminearumF. langsethiaeF. poaeF. pseudograminearumF. sporotrichioides
Alberta315494385120113414572871711
Manitoba94124281724511661084730116244
Eastern3435317341451392880029116
Saakatchewan25551263432125314246352097202831963
Team members

Research scientist/program manager

  • Dr. Sean Walkowiak

Biologists

  • Niradha Withana Gamage
  • Dr. Sung-Jong Lee
  • Dr. Tiffany Chin

Technicians

  • Janice Bamforth
  • Tehreem Ashfaq
  • Maria Eckhardt

Students

  • Mayantha Shimosh Kurera
  • Bhaktiben Patel
Recent publications
  • Borrill, P., Mago, R., Xu, T., Ford, B., Williams, S.J., Derkx, A., Bovill, W.D., Hyles, J., Bhatt, D., Xia, X., MacMillan, C., White, R., Buss, W., Molnár, I., Walkowiak, S., et al. 2022. An autoactive NB-LRR gene causes Rht13 dwarfism in wheat. Proc. Natl. Acad. Sci. U.S.A. 119(48): e2209875119.
    https://doi.org/10.1073/pnas.2209875119
  • Walkowiak, S., Taylor, D. Fu, B.X., Drul, D., Pleskach, K., and S.A. Tittlemier. 2022. Ergot in Canadian cereals – relevance, occurrence, and current status. Can. J. Plant Pathol. 44 (6): 793-805.
    https://doi.org/10.1080/07060661.2022.2077451
  • Chin, T., Pleskach, K., Tittlemier, S.A., Henriquez, M.A., Bamforth, J., Gamage, N.W., Ashfaq, T., Lee, S.-J., Kurera, M.S., Patel, B., and S. Walkowiak. 2023. A status update on fusarium head blight on Western Canadian wheat. Can. J. Plant Path.
    https://doi.org/10.1080/07060661.2023.2177352
  • Lee, S.-J., Demeke, T., Dusabenyagasani, M., Saydak, D., Perry, D. and S. Walkowiak. 2023. Evaluation of two high-throughput genotyping systems for rapid identification of Canadian wheat varieties. Can. J. Plant Sci.
    https://doi.org/10.1139/cjps-2022-0192
  • Bamforth, J., Chin, T., Ashfaq, T., Gamage, N.W., Pleskach, K., Tittlemier, S.A., Henriquez, M.A., Kurera, S., Lee, S.-J. Patel, B., Gräfenhan, T. and S. Walkowiak. 2023. A survey of Fusarium species and ADON genotype on Canadian wheat grain. Front. Fungal Bio. 3
    https://doi.org/10.3389/ffunb.2022.1062444

Page details

2023-08-02