Guidelines for Canadian Drinking Water Quality: Guideline Technical Document – Glyphosate

Exposure

Glyphosate and AMPA have not been detected in drinking water supplies. This may be because of the lack of monitoring studies and technical difficulties with available analytical methods.

Owing to their physicochemical properties, glyphosate and AMPA would be expected only in surface waters after direct application or after soil erosion due to precipitation, which could lead to contamination of downstream drinking water supplies. Experimental data indicate that glyphosate concentrations of 200-300 µg/L immediately after direct spraying in standing water drop to half this value in approximately three weeks ^{Footnote 1515} Direct spraying of glyphosate on lakes and ponds at 1 kg/ha resulted in an initial concentration of 1100 µg/L, dropping to 149 µg/L at two days and 55 µg/L after five days. ^{Footnote 99} In Quebec, the effect of glyphosate application for standard forestry practice (buffer zones of 30 m) was tested by monitoring eight streams and several ponds and drainage ditches. ^{Footnote 1616} None of the 60 samples at the eight streams contained detectable levels (detection limit 1.0 µg/L), although glyphosate was detected in two drainage ditch samples (maximum 16.9 µg/L). In the ponds, which were sprayed directly, the concentration was 2800 µg/L immediately post-spraying and fell to 288 µg/L after 24 hours.

Residues of glyphosate may occur in wheat and barley, products made from these grang beer ins (includi), lentils, soybeans and peas; concentrations in many cases are below or near the detection limit (0.05 mg/kg). The metabolite AMPA was only occasionally detected, usually at less than 2% of the total glyphosate residue; it was concluded that AMPA could be omitted from residue considerations. ^{Footnote 44 ,Footnote 1717} The theoretical maximum dietary intake of glyphosate was calculated to be about 2.7 µg/kg bw per day, based on 1986 data.^{Footnote 1818}

Analytical Methods and Treatment Technology

Glyphosate in water can be determined using direct aqueous injection high-pressure liquid chromatography (HPLC) with post-column derivatization. In this method, a small aliquot (200 µL) of filtered water is injected into a reverse-phase HPLC column. The post-column reactions involve oxidation of glyphosate to glycine, which then reacts with o-phthalaldehyde to form an isoindole that is measured fluorometrically. The detection limit for this method is 6 µg/L for tap-water and 9 µg/L for groundwater. ^{Footnote 1919}

Glyphosate can be removed from drinking water by conventional treatment. Bench-scale treatability studies ^{Footnote 20} indicate that oxidation, using chlorination or ozonation, is an effective treatment technique for glyphosate.

Health Effects

Pharmacokinetics

Glyphosate is incompletely absorbed (15-40%) by the oral route. ^{Footnote 2121} AMPA is also incompletely absorbed, at 20%. ^{Footnote 2222} Dermal absorption of glyphosate formulation (Roundup) in monkeys was only 2% after seven days of topical application. ^{Footnote 2323}

After oral or intraperitoneal administration of both a single dose and 12 days of repeated doses, glyphosate was excreted almost entirely in urine, largely in unchanged form, although small amounts of the metabolite AMPA were also found. Biliary excretion and enterohepatic circulation occurred only to a minor extent. ^{Footnote 44 ,Footnote 2121} Absorbed doses of AMPA were also excreted almost entirely via the urine, with less than 0.1% of the dose eliminated as labelled carbon dioxide. ^{Footnote 2222} Clearance of a single dose of glyphosate after 120 hours was 94% in males and females. With daily administration in the diet for 14 days, tissue levels reached maximum values by the sixth day of administration, with levels decreasing in the order kidneys (<1 ppm), spleen, fat, liver, ovaries, heart and muscle. Residues declined quickly once treatment ceased, with kidney levels dropping to 0.1 ppm at the end of a 10-day recovery period. ^{Footnote 2121}

Some concern has been expressed over the possibility that glyphosate could react with nitrite in the diet to form N-nitrosophosphonomethyl glycine (NPMG), a putative carcinogen. ^{Footnote 24}

Effects in Laboratory Animals and Humans

Glyphosate is acutely toxic to rats via the oral or dermal route at doses as low as 3800 mg/kg bw, with most values above 5000 mg/kg bw. ^{Footnote 2121} LD⁵⁰ values thus approach 1% of body weight. ^{Footnote 2525} There appear to be few toxic effects except body weight reduction and increased kidney weights at high doses. Glyphosate was only slightly irritating to skin and eyes when tested on 346 human volunteers, and there was no evidence of photoirritation or allergic or photoallergic contact dermatitis. ^{Footnote 2626} In animal tests for skin and eye irritation, glyphosate was classed as slightly irritating and the formulated product Roundup as moderately irritating. ^{Footnote 2525} Roundup was also more toxic (17-32 times) to fish than the parent compound, probably because of the amine salt or its surfactants. ^{Footnote 2525}

In a subchronic study, glyphosate was administered by gelatin capsule to beagle dogs (six per group) at doses of 0, 20, 100 or 500 mg/kg bw per day for one year. ^{Footnote 2727} There were no clinical signs of toxicity, and no treatment-related changes in body or organ weights or in ophthalmological, haematological, clinical chemistry or urinary parameters were observed. ^{Footnote 2727} A slightly increased incidence of lymphoid nodules was observed in the epididymis at the highest dose level, and a conservative NOAEL was considered to be 100 mg/kg bw per day.^{Footnote 28}

In a chronic toxicity/carcinogenicity study carried out with CD-1 mice (50 per sex per group), glyphosate was administered in the diet at 0, 0.1, 0.5 or 3% (0, 1000, 5000 and 30 000 ppm) for 24 months. ^{Footnote 2929} In high-dose males, a slight increase in liver necrosis (hepatic hypertrophy and centrilobular necrosis), some interstitial kidney nephrosis and hyperplasia of the urinary bladder were observed. Slight decreases in body weight gain were noted in both males and females at the high dose. There were no other treatment-related changes in body or organ weights, food and water consumption or haematological parameters, and there were no clinical signs of toxicity at any dose levels. The NOAEL was considered to be 5000 ppm, equivalent to 814 mg/kg bw per day for males. ^{Footnote 29} There were no significant increases in the frequency of neoplastic lesions, although three small renal tubule adenomas were observed in high-dose males; their incidence was below that in the historical controls. Glyphosate was not considered to be tumorigenic to mice in this study. ^{Footnote 2121,Footnote 30}

In a two-year feeding/oncogenicity study, Sprague-Dawley rats (50 per sex per dose) were administered technical glyphosate at 0, 60, 200 and 600 ppm in the diet (equivalent to intakes of 0, 3.0, 10.3 or 31.4 mg/kg bw per day for males and 0, 3.4, 11.2 or 34 mg/kg bw per day for females).^{Footnote 31} There was a consistent tendency for reduced body weight gains in treated males during most of the growth period, but effects on body weight were not considered to be treatment-related. ^{Footnote 2121} No other adverse effects were observed on mortality, food and water intake, absolute or relative organ weights, haematology, clinical chemistry or urinalysis. The incidence of interstitial cell testicular tumours was slightly elevated in low- and high-dose groups; however, it was not dose-related, it was close to historical control values and it was deemed unlikely to be treatment-related. The highest dose (31.4 or 34 mg/kg bw per day) was considered to be the no-observed-adverse-effect level (NOAEL for this study. ^{Footnote 2121,Footnote 3131,Footnote 3232} Equivocal evidence of a slightly increased incidence of thyroid C-cell carcinomas was noted in high-dose females by Health Canada toxicologists. Because of concern over this finding, the lowest dose of 3.0 mg/kg bw per day was suggested as the NOAEL, based on the trend to body weight gain reductions seen in males. ^{Footnote 1818Footnote *}

In a repeat study, the same strain of rats was administered glyphosate in the diet at higher doses of 0, 2000, 8000 or 20 000 ppm for two years.^{,Footnote 3333} At the highest dose level (20 000 ppm, or approximately 1000 mg/kg bw per day), body weight and body weight gains were reduced in females, increased liver weights, cataractogenic effects (degenerative lens changes) and testicular effects (not further defined) were noted in males, and an increased incidence of stomach irritation was observed in both sexes (statistically significant in females). One evaluation^{Footnote 2828} reported no indication of any tumorigenic activity at any dose; however, a second evaluation ^{Footnote 6} stated that the incidence of adenomas of the pancreatic islet cells was increased in low- and high-dose males but was not dose-related, and the trend test was negative. No pancreatic carcinomas were observed. The NOAEL was considered to be 8000 ppm, equivalent to 362 mg/kg bw per day.^{Footnote 2828,Footnote 3333}

Glyphosate was not mutagenic in a battery of in vitro and in vivo short-term tests, including the Ames test, with and without metabolic activation, on four strains of Salmonella typhimurium, a recombination assay with Bacillus subtilis H17(rec+) and M45(rec-), a reverse mutation test with Escherichia coli WP2her, a forward mutation assay with Chinese hamster ovary cells and an unscheduled DNA repair assay using rat hepatocytes. Negative in vivo tests included a mouse dominant lethal assay at oral doses of 200-1000 mg/kg bw and a host-mediated assay on rat bone marrow at intraperitoneal doses of 200-1000 mg/kg. ^{Footnote 21,Footnote 34}

No teratogenic effects were observed in rabbit and rat studies at doses as high as 350 and 3500 mg/kg bw per day, respectively. In the rat study (25 per group), gavage dose levels were 0, 300, 1000 and 3500 mg/kg bw. Severe maternal toxicity was observed at the high dose, with diarrhoea, red nasal discharge, reduced body weight gain and six deaths by day 17. Reproduction, including total implants, viable foetuses and resorptions, was adversely affected at this dose level, and foeto-toxicity was evident, with observations of unossified sternebrae and kidney anomalies (three pups). ^{Footnote 2121,Footnote 3434} In the rabbit study, groups of 16 females received 0, 75, 175 and 350 mg/kg bw per day by gavage on days 6-27 of gestation. Maternal toxicity was apparent at both middle and high dose levels (diarrhoea, nasal discharge, transient body weight reduction and deaths in two and 10 females from the mid- and high-dose groups, respectively). Treatment-related effects on the number of viable foetuses and on early or late resorptions and an increased incidence of skeletal variation were noted in the high-dose group. ^{Footnote 2121 ,Footnote 3434} The NOAELs for maternal and foetal toxicity were 75 and 175 mg/kg bw per day, respectively.

No adverse reproductive effects were noted in a two-litter, three-generation rat study in which groups of 12 male and 24 female S-D rats were given glyphosate in the diet at dose levels of 0, 3, 10 or 30 mg/kg bw for 60 days, continued throughout mating, gestation and lactation, for three generations. The no-observed-effect-level (NOEL) was considered to be at or above the highest dose level of 30 mg/kg bw. ^{Footnote 821}

Rationale

Based on evaluations by the Food Directorate of Health Canada,^{Footnote 18} a negligible daily intake (NDI) for glyphosate was established as follows:

where:

• 3.0 mg/kg bw per day was the NOAEL for reduced body weight gain in a two-year rat feeding/oncogenicity study ^{Footnote 3030}
• 100 is the uncertainty factor (×10 for interspecies variation and ×10 for intraspecies variation in an adequate long-term study).

The maximum acceptable concentration (MAC) for glyphosate in drinking water is derived from the NDI as follows:

where:

• 0.03 mg/kg bw per day is the NDI established by the Food Directorate of Health Canada
• 70 kg is the average body weight of an adult
• 0.20 is the proportion of total daily intake of glyphosate allocated to water (theoretical maximum intake from food is 9% of the NDI)
• 1.5 L/d is the average daily consumption of drinking water for an adult.

References

^{Footnote *} A later independent analysis by three pathologists discounted the relationship between treatment and the C-cell tumours. ^{Footnote 2828}