Biological test methods publications

If you require a method for which a link is not provided, please email your request to ec.methodes-methods.ec@canada.ca.

Biological test methods and guidance documents are amended periodically through the publication of amendment sheets to be used in conjunction with existing methods, or through the publication of subsequent editions. In recent documents, amendments have been highlighted in green.

This table describes biological test methods and guidance documents available via email. It provides a thumbnail image of the report cover and lists each report title, publication date with amendments, and reference number.

Biological Test Methods and Guidance Documents
Report Title	Publication Date	Reference
Biological Test Method: Acute Lethality Test Using Rainbow Trout HTML Version	July 1990, Amendments: May 1996, May 2007	EPS1/RM/09
Biological Test Method: Acute Lethality Test Using Threespine Stickleback (Gasterosteus aculeatus)	Second Edition: December 2017	EPS1/RM/10
Biological Test Method: Acute Lethality Test Using Daphnia spp. HTML Version	July 1990, Amendments: May 1996	EPS1/RM/11
Guidance Document on Control of Toxicity Test Precision Using Reference Toxicants	August 1990	EPS1/RM/12
Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Rainbow Trout HTML Version	Second Edition: December 2000, Amendments: May 2007 and February 2016	EPS1/RM/13
Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Daphnia magna HTML Version	Second Edition: December 2000, Amendments: February 2016	EPS1/RM/14
Biological Test Method: Test of Reproduction and Survival Using the Cladoceran Ceriodaphnia dubia HTML Version	Second Edition: February 2007	EPS1/RM/21
Biological Test Method: Test of Larval Growth and Survival Using Fathead Minnows HTML Version	Second Edition: February 2011	EPS1/RM/22
Biological Test Method: Toxicity Test Using Luminescent Bacteria	November 1992	EPS1/RM/24
Biological Test Method: Growth Inhibition Test Using a Freshwater Alga HTML Version	Second Edition: March 2007	EPS1/RM/25
Biological Test Method: Acute Test for Sediment Toxicity Using Marine or Estuarine Amphipods	December 1992, Amendments: October 1998	EPS1/RM/26
Biological Test Method: Fertilization Assay Using Echinoids (Sea Urchins and Sand Dollars) HTML Version	Second Edition: February 2011	EPS1/RM/27
Biological Test Method: Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout) HTML Version	Second Edition: July 1998	EPS1/RM/28
Guidance Document on Collection and Preparation of Sediments for Physicochemical Characterization and Biological Testing	December 1994	EPS1/RM/29
Guidance Document on Measurement of Toxicity Test Precision Using Control Sediments Spiked with a Reference Toxicant	September 1995	EPS1/RM/30
Biological Test Method: Test for Survival and Growth in Sediment Using Larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius)	December 1997	EPS1/RM/32
Biological test method: test for survival, growth and reproduction in sediment and water using the freshwater amphipod Hyalella azteca	Third Edition: September 2017	EPS1/RM/33
Guidance Document on Application and Interpretation of Single-species Tests in Environmental Toxicology	December 1999	EPS1/RM/34
Biological Test Method: Reference Method for Determining Acute Lethality of Sediment to Marine or Estuarine Amphipods HTML Version	December 1998	EPS1/RM/35
Biological Test Method: Test for Measuring the Inhibition of Growth Using the Freshwater Macrophyte, Lemna minor	Second Edition: January 2007	EPS1/RM/37
Biological Test Method: Test for Survival and Growth in Sediment Using Spionid Polychaete Worms (Polydora cornuta)	December 2001	EPS1/RM/41
Biological Test Method: Reference Method for Determining the Toxicity of Sediment Using Luminescent Bacteria in a Solid-Phase Test	April 2002	EPS1/RM/42
Biological Test Method: Tests for Toxicity of Contaminated Soil to Earthworms (Eiseniaandrei, Eisenia fetida or Lumbricus terrestris)^Footnote1	June 2004, Amendments: June 2007	EPS1/RM/43
Guidance Document for Testing the Pathogenicity and Toxicity of New Microbial Substances to Aquatic and Terrestrial Organisms	Second Edition: December 2016	EPS1/RM/44
Biological Test Method: Test for Measuring Emergence and Growth of Terrestrial Plants Exposed to Contaminants in Soil^Footnote1	February 2005, Amendments: June 2007	EPS1/RM/45
Guidance Document on Statistical Methods for Environmental Toxicity Tests^Footnote1	March 2005, Amendments: June 2007	EPS1/RM/46
Biological Test Method: Test for Measuring Survival and Reproduction of Springtails Exposed to Contaminants in Soil^{Footnote 1,} ^Footnote2	February 2014	EPS1/RM/47
Procedure for pH Stabilization During the Testing of Acute Lethality of Wastewater Effluent to Rainbow Trout	March 2008	EPS1/RM/50
Supplementary Background and Guidance for Investigating Acute Lethality of Wastewater Effluent to Rainbow Trout	March 2008	N/A
Guidance Document on the Sampling and Preparation of Contaminated Soil for Use in Biological Testing	2012	EPS1/RM/53
Biological Test Method: Test for Growth in Contaminated Soil Using Terrestrial Plants Native to the Boreal Region	2013	EPS1/RM/56
Reference method for measuring the toxicity of contaminated sediment to embryos and larvae of echinoids (sea urchins or sand dollars) HTML version	July 2014	1/RM/58
Procedure for pH stabilization during the testing of acute lethality of pulp and paper effluent to Rainbow Trout	June 2018	STB 1/RM/59
Biological test method : reference method for determining acute lethality using Acartia tonsa	June 2019	STB 1/RM/60
Biological test method: test for measuring reproduction of oribatid mites exposed to contaminants in soil	September 2020	STB 1/RM/61

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