Biological test methods publications

If you require a method for which a link is not provided, please email your request to ec.methodes-methods.ec@canada.ca.

Biological test methods and guidance documents are amended periodically through the publication of amendment sheets to be used in conjunction with existing methods, or through the publication of subsequent editions. In recent documents, amendments have been highlighted in green.

This table describes biological test methods and guidance documents available via email. It provides a thumbnail image of the report cover and lists each report title, publication date with amendments, and reference number.

Biological Test Methods and Guidance Documents
Report Title Publication Date Reference

Biological Test Method: Acute Lethality Test Using Rainbow Trout

HTML Version

July 1990, Amendments: May 1996, May 2007 EPS1/RM/09
Biological Test Method: Acute Lethality Test Using Threespine Stickleback (Gasterosteus aculeatus) Second Edition: December 2017 EPS1/RM/10

Biological Test Method: Acute Lethality Test Using Daphnia spp.

HTML Version

July 1990, Amendments: May 1996 EPS1/RM/11
Guidance Document on Control of Toxicity Test Precision Using Reference Toxicants August 1990 EPS1/RM/12

Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Rainbow Trout

HTML Version

Second Edition: December 2000, Amendments: May 2007 and February 2016 EPS1/RM/13

Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Daphnia magna

HTML Version

Second Edition: December 2000, Amendments: February 2016 EPS1/RM/14

Biological Test Method: Test of Reproduction and Survival Using the Cladoceran Ceriodaphnia dubia

HTML Version

Second Edition: February 2007 EPS1/RM/21

Biological Test Method: Test of Larval Growth and Survival Using Fathead Minnows

HTML Version

Second Edition: February 2011 EPS1/RM/22
Biological Test Method: Toxicity Test Using Luminescent Bacteria November 1992 EPS1/RM/24

Biological Test Method: Growth Inhibition Test Using a Freshwater Alga

HTML Version

Second Edition: March 2007 EPS1/RM/25
Biological Test Method: Acute Test for Sediment Toxicity Using Marine or Estuarine Amphipods December 1992, Amendments: October 1998 EPS1/RM/26

Biological Test Method: Fertilization Assay Using Echinoids (Sea Urchins and Sand Dollars)

HTML Version

Second Edition: February 2011 EPS1/RM/27

Biological Test Method: Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout)

HTML Version

Second Edition: July 1998 EPS1/RM/28
Guidance Document on Collection and Preparation of Sediments for Physicochemical Characterization and Biological Testing December 1994 EPS1/RM/29
Guidance Document on Measurement of Toxicity Test Precision Using Control Sediments Spiked with a Reference Toxicant September 1995 EPS1/RM/30
Biological Test Method: Test for Survival and Growth in Sediment Using Larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius) December 1997 EPS1/RM/32
Biological test method: test for survival, growth and reproduction in sediment and water using the freshwater amphipod Hyalella azteca Third Edition: September 2017 EPS1/RM/33
Guidance Document on Application and Interpretation of Single-species Tests in Environmental Toxicology December 1999 EPS1/RM/34

Biological Test Method: Reference Method for Determining Acute Lethality of Sediment to Marine or Estuarine Amphipods

HTML Version

December 1998 EPS1/RM/35
Biological Test Method: Test for Measuring the Inhibition of Growth Using the Freshwater Macrophyte, Lemna minor Second Edition: January 2007 EPS1/RM/37
Biological Test Method: Test for Survival and Growth in Sediment Using Spionid Polychaete Worms (Polydora cornuta) December 2001 EPS1/RM/41
Biological Test Method: Reference Method for Determining the Toxicity of Sediment Using Luminescent Bacteria in a Solid-Phase Test April 2002 EPS1/RM/42
Biological Test Method: Tests for Toxicity of Contaminated Soil to Earthworms (Eiseniaandrei, Eisenia fetida or Lumbricus terrestris)Footnote1 June 2004, Amendments: June 2007 EPS1/RM/43
Guidance Document for Testing the Pathogenicity and Toxicity of New Microbial Substances to Aquatic and Terrestrial Organisms Second Edition: December 2016 EPS1/RM/44
Biological Test Method: Test for Measuring Emergence and Growth of Terrestrial Plants Exposed to Contaminants in SoilFootnote1 February 2005, Amendments: June 2007 EPS1/RM/45
Guidance Document on Statistical Methods for Environmental Toxicity TestsFootnote1 March 2005, Amendments: June 2007 EPS1/RM/46
Biological Test Method: Test for Measuring Survival and Reproduction of Springtails Exposed to Contaminants in SoilFootnote 1, Footnote2 February 2014 EPS1/RM/47
Procedure for pH Stabilization During the Testing of Acute Lethality of Wastewater Effluent to Rainbow Trout March 2008 EPS1/RM/50
Supplementary Background and Guidance for Investigating Acute Lethality of Wastewater Effluent to Rainbow Trout March 2008 N/A
Guidance Document on the Sampling and Preparation of Contaminated Soil for Use in Biological Testing 2012 EPS1/RM/53
Biological Test Method: Test for Growth in Contaminated Soil Using Terrestrial Plants Native to the Boreal Region 2013 EPS1/RM/56
Reference method for measuring the toxicity of contaminated sediment to embryos and larvae of echinoids (sea urchins or sand dollars) July 2014 1/RM/58
Procedure for pH stabilization during the testing of acute lethality of pulp and paper effluent to Rainbow Trout June 2018 STB 1/RM/59
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