Biological test method for measuring survival of springtails exposed to contaminants in soil
Table of Contents
- Abstract
- Foreword
- Terminology
- Acknowledgements
- Section 1 - Introduction
- Section 2 - Test Organisms
- Section 3 - Test System
- Section 4 - Universal Test Procedures
- Section 5 - Specific Procedures for Testing Field-collected Soil or Similar Particulate Material
- Section 6 - Specific Procedures for Testing Chemical-spiked Soil
- Section 7 - Reporting Requirements
- References
- Appendix A - Biological Test Methods and Supporting Guidance Documents Published by Environment Canada’s Method Development and Applications Unit
- Appendix B - Environment Canada Regional and Headquarters Offices
- Appendix C - Members of the Inter-Governmental Ecotoxicological Testing Group (as of April 2013)
- Appendix D - Members of the Scientific Advisory Group
- Appendix E - Procedural Variations for Culturing Springtails, as Described in International Guides and Test Methods for Measuring Soil Toxicity Using Various Species of Springtails
- Appendix F - Procedural Variations for Tests of Effects of Contaminated Soil on the Survival and Reproduction of Springtails, as Described in International Methodology Documents
- Appendix G - Natural and Artificial Negative Control Soils Used for Method Development and the Establishment of Test Validity Criteria
- Appendix H - Logarithmic Series of Concentrations Suitable for Toxicity Tests
- Appendix I - Aspirator Construction and Usage
EPS 1/RM/47 Second edition - February 2014
Science and Technology Branch
Environment Canada
Environmental Protection Series
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- Research and Technology Development
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Subject Areas
- AG
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Agriculture
- AN
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Anaerobic Technology
- AP
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Airborne Pollutants
- AT
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Aquatic Toxicity
- CC
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Commercial Chemicals
- CE
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Consumers and the Environment
- CI
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Chemical Industries
- FA
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Federal Activities
- FP
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Food Processing
- HA
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Hazardous Wastes
- IC
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Inorganic Chemicals
- MA
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Marine Pollutants
- MM
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Mining and Ore Processing
- NR
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Northern and Rural Regions
- PF
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Paper and Fibres
- PG
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Power Generation
- PN
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Petroleum and Natural Gas
- RA
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Refrigeration and Air Conditioning
- RM
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Reference Methods
- SF
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Surface Finishing
- SP
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Oil and Chemical Spills
- SRM
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Standard Reference Methods
- TS
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Transportation Systems
- TX
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Textiles
- UP
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Urban Pollution
- WP
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Wood Protection/Preservation
New subject areas and codes are introduced as they become necessary. A list of EPS reports may be obtained from Communications Services, Environment Canada, Ottawa, Ontario, K1A 0H3, Canada.
Method Development and Applications Unit
Science and Technology Branch
Environment Canada
Ottawa, Ontario
Report EPS 1/RM/47
Second Edition
February 2014
Readers’ Comments
Comments regarding the content of this report should be addressed to:
Richard Scroggins, Chief
Biological Assessment and Standardization Section
Science and Technology Branch
Environment Canada
335 River Road
Ottawa, Ontario
K1A 0H3
Lisa Taylor, Manager
Method Development and Applications Unit
Science and Technology Branch
Environment Canada
335 River Road
Ottawa, Ontario
K1A 0H3
Cette publication est aussi disponible en français. Pour l'obtenir, s'adresser à:
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Environnement Canada
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K1A 0H3
Review Notice
This report has been reviewed by staff from the Science and Technology Branch, Environment Canada, and approved for publication. Mention of trade names or commercial products does not constitute endorsement by Environment Canada for use. Other products of similar value are available.
List of Abbreviations and Chemical Formulae
- AES
- atomic emission spectrophotometry
- mm
- millimetre(s)
- Al
- aluminum
- mS
- millisiemens
- ANOVA
- analysis of variance
- n
- sample size
- CaCl 2
- calcium chloride
- N
- nitrogen
- CaCO 3
- calcium carbonate
- Na
- sodium
- Ca(OH) 2
- calcium hydroxide
- NH 4
- ammonium
- CCME
- Canadian Council of Ministers of the Environment
- nm
- nanometre(s)
- NOEC
- no-observed-effect concentration
- C
- carbon
- OM
- organic matter
- Ca
- calcium
- p
- probability
- CEC
- cation exchange capacity
- PACs
- polycyclic aromatic compounds
- Cl
- chlorine
- PAHs
- polycyclic aromatic hydrocarbons
- cm
- centimetre(s)
- PAO
- post antennal organ
- CV
- coefficient of variation
- QA/QC
- quality assurance/quality control
- °C
- degree(s) Celcius
- SD
- standard deviation
- d
- day(s)
- S
- sulphur
- DDT
- dichloro-diphenyl-trichloroethane
- s
- second
- diam
- diameter
- sp.
- species (singular)
- DQO
- data quality objective
- spp.
- species (plural)
- ECp
- effect concentration (for a specified percent)
- t
- time
- ERA
- ecological risk assessment
- TOC
- total organic carbon
- TM ( TM)
- Trade Mark
- ES
- effect size
- v:m
- volume-to-mass
- g
- gram(s)
- v:v
- volume-to-volume
- h
- hour(s)
- W
- Watt
- H 3BO 3
- boric acid
- WHC
- water-holding capacity
- HCl
- hydrochloric acid
- wt
- weight
- HNO 3
- nitric acid
- α
- level of statistical significance (alpha)
- HPLC
- high performance liquid chromatography
- μg
- microgram(s)
- μm
- micrometre(s)
- H 2O
- water
- μmhos
- micromhos
- H 0
- null hypothesis
- μmol
- micromole(s)
- ICAP
- inductively coupled argon plasma
- >
- greater than
- ICp
- inhibiting concentration (for a specified percent effect)
- <
- less than
- ≥
- greater than or equal to
- K
- potassium
- ≤
- less than or equal to
- KCl
- potassium chloride
- %
- percentage or percent
- kg
- kilogram(s)
- =
- equals
- L
- litre(s)
- +
- plus
- LAS
- linear alkyl benzene sulphonates
- -
- minus
- LC50
- median lethal concentration
- ±
- plus or minus
- LOEC
- lowest-observed-effect concentration
- X
- times
- m
- metre(s)
- ÷
- divided by
- M
- mole(s) (concentration)
- /
- per; alternatively, “or” (e.g., survival/reproduction)
- Mg
- magnesium
- mg
- milligram(s)
- ≈
- approximately equal to
- mL
- millilitre(s)
- ~
- approximately
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