Biological test method for measuring terrestrial plants exposed to contaminants in soil: appendix A


Appendix A - Biological Test Methods and Supporting Guidance Documents Published by Environment Canada's Method Development and Applications Section

A. Generic (Universal) Biological Test Methods
Title of Biological Test Method or Guidance Document Report Number Publication Date Applicable Amendments
Acute Lethality Test Using Rainbow Trout EPS 1/RM/9 July 1990 May 1996
Acute Lethality Test Using Threespine Stickleback (Gasterosteus aculeatus) EPS 1/RM/10 July 1990 March 2000
Acute Lethality Test Using Daphnia spp. EPS 1/RM/11 July 1990 May 1996
Test of Reproduction and Survival Using the Cladoceran Ceriodaphnia dubia EPS 1/RM/21 February 1992 November 1997
Test of Larval Growth and Survival Using Fathead Minnows EPS 1/RM/22 February 1992 November 1997
Toxicity Test Using Luminescent Bacteria (Photobacterium phosphoreum) EPS 1/RM/24 November 1992 --
Growth Inhibition Test Using the Freshwater Alga Selenastrum capricornutum EPS 1/RM/25 November 1992 November 1997
Acute Test for Sediment Toxicity Using Marine or Estuarine Amphipods EPS 1/RM/26 December 1992 October 1998
Fertilization Assay Using Echinoids (Sea Urchins and Sand Dollars) EPS 1/RM/27 December 1992 November 1997
Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout, Coho Salmon, or Atlantic Salmon) EPS 1/RM/28 1st Edition December 1992 January 1995
Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout) EPS 1/RM/28 2nd  Edition July 1998 --
Test for Survival and Growth in Sediment Using the Larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius) EPS 1/RM/32 December 1997 --
Test for Survival and Growth in Sediment Using the Freshwater Amphipod Hyalella azteca EPS 1/RM/33 December 1997 --
Test for Measuring the Inhibition of Growth Using the Freshwater Macrophyte, Lemna minor EPS 1/RM/37 March 1999 --
Test for Survival and Growth in Sediment Using
Spionid Polychaete Worms (Polydora cornuta)
EPS 1/RM/41 December 2001 --
Tests for Toxicity of Contaminated Soil to Earthworms (Eisenia andrei, Eisenia fetida, or Lumbricus terrestris) EPS 1/RM/43 June 2004 --
Tests for Measuring Emergence and Growth of
Terrestrial Plants Exposed to Contaminants in Soil
EPS 1/RM/45 February 2005 --
Test for Measuring Survival and Reproduction of
Springtails Exposed to Contaminants in Soil
EPS 1/RM/47 2005 --
B. Reference Methods**
Title of Biological Test Method or Guidance Document Report Number Publication Date Applicable Amendments
Reference Method for Determining Acute Lethality of Effluents to Rainbow Trout EPS 1/RM/13 1st Edition July 1990 May 1996, December 2000
Reference Method for Determining Acute Lethality of Effluents to Rainbow Trout EPS 1/RM/13 2nd  Edition December 2000 --
Reference Method for Determining Acute Lethality of Effluents to Daphnia magna EPS 1/RM/14 1st Edition July 1990 May 1996, December 2000
Reference Method for Determining Acute Lethality of Effluents to Daphnia magna EPS 1/RM/14 2nd Edition December 2000 --
Reference Method for Determining Acute Lethality of Sediment to Marine or Estuarine Amphipods EPS 1/RM/35 December 1998 --
Reference Method for Determining the Toxicity of Sediment Using Luminescent Bacteria in a Solid- Phase Test EPS 1/RM/42 April 2002 --
C. Supporting Guidance Documents
Title of Biological Test Method or Guidance Document Report Number Publication Date Applicable Amendments
Guidance Document on Control of Toxicity Test Precision Using Reference Toxicants EPS 1/RM/12 August 1990 --
Guidance Document on Collection and Preparation of Sediment for Physicochemical Characterization and Biological Testing EPS 1/RM/29 December 1994 --
Guidance Document on Measurement of Toxicity Test Precision Using Control Sediments Spiked with a Reference Toxicant EPS 1/RM/30 September 1995 --
Guidance Document on Application and Interpretation of Single-Species Tests in Environmental Toxicology EPS 1/RM/34 December 1999 --
Guidance Document for Testing the Pathogenicity and Toxicity of New Microbial Substances to Aquatic and Terrestrial Organisms EPS 1/RM/44 March 2004 --
Guidance Document on Statistical Methods to Determine Endpoints of Toxicity Tests EPS 1/RM/46 December 2004 --

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