Biological test method for measuring the inhibition of growth using freshwater macrophyte: contents

Table of Contents

• Front Matter
  • Readers' Comments
  • Review Notice
  • Abstract
  • Foreword
  • List of Abbreviations and Chemical Formulae
  • Terminology
  • Acknowledgements
• Section 1: Introduction
  • 1.1 Background
  • 1.2 Species Description and Historical Use in Tests
• Section 2: Test Organisms
  • 2.1 Species and Life Stage
  • 2.2 Source
  • 2.3 Culturing
    • 2.3.1 General
    • 2.3.2 Facilities and Apparatus
    • 2.3.3 Growth Medium
    • 2.3.4 Lighting
    • 2.3.5 Temperature
    • 2.3.6 pH
    • 2.3.7 Culture Maintenance
    • 2.3.8 Health Criteria
• Section 3: Test System
  • 3.1 Facilities and Apparatus
  • 3.2 Lighting
  • 3.3 Test Vessels
  • 3.4 Control/Dilution Water
• Section 4: Universal Test Procedures
  • 4.1 Preparing Test Solutions
  • 4.2 Beginning the Test
  • 4.3 Test Conditions
    • 4.3.1 pH
  • 4.4 Test Observations and Measurements
  • 4.5 Test Endpoints and Calculations
    • 4.5.1 Validity of Test
    • 4.5.2 Multi-Concentration Tests
    • 4.5.3 Single Concentration Tests
    • 4.5.4 Stimulatory Effect
    • 4.5.5 Other Test Designs and Purposes
  • 4.6 Reference Toxicant
  • 4.7 Legal Considerations
• Section 5: Specific Procedures for Testing Chemicals
  • 5.1 Properties, Labelling, and Storage of Sample
  • 5.2 Preparing Test Solutions
  • 5.3 Control/Dilution Water
  • 5.4 Test Observations and Measurements
  • 5.5 Test Endpoints and Calculations
  • 5.6 Interpretation of Results
• Section 6: Specific Procedures for Testing Samples of Effluent, Leachate, and Elutriate
  • 6.1 Sample Collection, Labelling, Transport, and Storage
  • 6.2 Preparing Test Solutions
  • 6.3 Control/Dilution Water
  • 6.4 Test Observations and Measurements
  • 6.5 Test Endpoints and Calculations
  • 6.6 Interpretation of Results
• Section 7: Specific Procedures for Testing Receiving-Water Samples
  • 7.1 Sample Collection, Labelling, Transport, and Storage
  • 7.2 Preparing Test Solutions
  • 7.3 Control/Dilution Water
  • 7.4 Test Observations and Measurements
  • 7.5 Test Endpoints and Calculations
• Section 8: Reporting Requirements
  • 8.1 Minimum Requirements for a Test-Specific Report
    • 8.1.1 Test Substance
    • 8.1.2 Test Organisms
    • 8.1.3 Test Facilities and Apparatus
    • 8.1.4 Control/Dilution Water
    • 8.1.5 Test Method
    • 8.1.6 Test Conditions and Procedures
    • 8.1.7 Test Results
  • 8.2 Additional Reporting Requirements
    • 8.2.1 Test Substance
    • 8.2.2 Test Organisms
    • 8.2.3 Test Facilities and Apparatus
    • 8.2.4 Control/Dilution Water
    • 8.2.5 Test Method
    • 8.2.6 Test Conditions and Procedures
    • 8.2.7 Test Results
• References
• Appendices
  1. Members of the Inter-Governmental Environmental Toxicity Group (as of December 2006)
  2. Environment Canada, Environmental Protection Service, Regional and Headquarters Offices
  3. Procedural Variations for Culturing Lemna spp., and for Undertaking Growth Inhibition Tests Using Lemna spp., as Described in Canadian, American, and European Methodology Documents
  4. Review of Culture and Test Media Used in Lemna spp. Growth Inhibition Tests, as Described in Canadian, American, and European Methodology Documents
  5. General Description of Lemna minor
  6. Axenic Culture Techniques for Lemna (Acreman, 2006)
  7. Logarithmic Series of Concentrations Suitable for Toxicity Tests
  8. Biological Test Methods and Supporting Guidance Documents Published by Environment Canada’s Method Development & Applications Section

List of Tables

1. Checklist of Recommended Conditions and Procedures for Culturing Lemna minor
2. Chemical Composition of Nutrient Stock Solutions for Preparing Modified Hoagland’s E+ Medium (Saskatchewan Research Council (SRC), 2003), Used for Culturing Lemna minor
3. Checklist of Recommended Test Conditions and Procedures for Conducting Toxicity Tests Using Lemna minor
4. Chemical Composition of Nutrient Stock Solutions for Preparing Swedish Standards Institute (SIS) Medium and Nutrient-Spiked Receiving Water, for Testing Chemical Samples Using Lemna minor
5. Chemical Composition of Nutrient Stock Solutions for Preparing Modified American Public Health Association (APHA) Medium, Nutrient-Spiked Wastewater, and Nutrient-Spiked Receiving Water, for Testing Samples of Effluent, Elutriate, Leachate, or Receiving Water, Using Lemna minor

List of Figures

1. Considerations for Preparing and Performing Toxicity Tests Using Lemna minor with Various Types of Test Materials or Substances
2. General Appearance of Healthy and Unhealthy Lemna minor