Biological test method for measuring survival of springtails exposed to contaminants in soil: appendix A


Appendix A

Biological Test Methods and Supporting Guidance Documents Published by Environment Canada’s Method Development and Applications Unit.Footnotea

A. Generic (Universal) Biological Test Methods
Title of Biological Test Method or Guidance Document Report Number Publication Date Applicable Amendments
Acute Lethality Test Using Rainbow Trout EPS 1/RM/9 July 1990 May 1996 and May 2007
Acute Lethality Test Using Threespine Stickleback
(Gasterosteus aculeatus)
EPS 1/RM/10 July 1990 March 2000
Acute Lethality Test Using Daphnia spp. EPS 1/RM/11 July 1990 May 1996
Test of Reproduction and Survival Using the Cladoceran Ceriodaphnia dubia EPS 1/RM/21
2nd Edition
February 2007 -
Test of Larval Growth and Survival Using Fathead Minnows EPS 1/RM/22
2nd Edition
February 2011 -
Toxicity Test Using Luminescent Bacteria
(Photobacterium phosphoreum)
EPS 1/RM/24 November 1992 -
Growth Inhibition Test Using a Freshwater Algae EPS 1/RM/25
2nd Edition
March 2007 -
Acute Test for Sediment Toxicity Using Marine or Estuarine Amphipods EPS 1/RM/26 December 1992 October 1998
Fertilization Assay Using Echinoids (Sea Urchins and Sand Dollars) EPS 1/RM/27
2nd Edition
February 2011 -
Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout) EPS 1/RM/28
2nd Edition
July 1998 -
Test for Survival and Growth in Sediment Using the Larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius) EPS 1/RM/32 December 1997 -
Test for Survival and Growth in Sediment and Water Using the Freshwater Amphipod Hyalella azteca EPS 1/RM/33
2nd Edition
June 2012 -
Test for Measuring the Inhibition of Growth Using the Freshwater Macrophyte, Lemna minor EPS 1/RM/37
2nd Edition
January 2007 -
Test for Survival and Growth in Sediment Using Spionid Polychaete Worms (Polydora cornuta) EPS 1/RM/41 December 2001 -
Tests for Toxicity of Contaminated Soil to Earthworms (Eisenia andrei, Eisenia fetida, or Lumbricus terrestris) EPS 1/RM/43 June 2004 June 2007
Tests for Measuring Emergence and Growth of Terrestrial Plants Exposed to Contaminants in Soil EPS 1/RM/45 February 2005 June 2007
Test for Measuring Survival and Reproduction of Springtails Exposed to Contaminants in Soil EPS 1/RM/47 September 2007 -
Test for Growth in Contaminated Soil Using Terrestrial Plants Native to the Boreal Region EPS 1/RM/56 August 2013 -
B. Reference Methods Footnoteb
Title of Biological Test Method or Guidance Document Report Number Publication Date Applicable Amendments
Reference Method for Determining Acute Lethality of Effluents to Rainbow Trout EPS 1/RM/13
2nd Edition
December 2000 May 2007
Reference Method for Determining Acute Lethality of Effluents to Daphnia magna EPS 1/RM/14
2nd Edition
December 2000 -
Reference Method for Determining Acute Lethality of Sediment to Marine or Estuarine Amphipods EPS 1/RM/35 December 1998 -
Reference Method for Determining the Toxicity of Sediment Using Luminescent Bacteria in a Solid- Phase Test EPS 1/RM/42 April 2002 -
C. Supporting Guidance Documents
Title of Biological Test Method or Guidance Document Report Number Publication Date Applicable Amendments
Guidance Document on Control of Toxicity Test Precision Using Reference Toxicants EPS 1/RM/12 August 1990 -
Guidance Document on Collection and Preparation of Sediment for Physicochemical Characterization and Biological Testing EPS 1/RM/29 December 1994 -
Guidance Document on Measurement of Toxicity Test Precision Using Control Sediments Spiked with a Reference Toxicant EPS 1/RM/30 September 1995 -
Guidance Document on Application and Interpretation of Single-Species Tests in Environmental Toxicology EPS 1/RM/34 December 1999 -
Guidance Document for Testing the Pathogenicity and Toxicity of New Microbial Substances to Aquatic and Terrestrial Organisms EPS 1/RM/44 March 2004 -
Guidance Document on Statistical Methods for Environmental Toxicity Tests EPS 1/RM/46 March 2005 June 2007
Procedure for pH Stabilization During the Testing of Acute Lethality of Wastewater Effluent to Rainbow Trout EPS 1/RM/50 March 2008 -
Supplementary Background and Guidance for Investigating Acute Lethality of Wastewater Effluent to Rainbow Trout - March 2008 -
Guidance Document on the Sampling and Preparation of Contaminated Soil for Use in Biological Testing EPS 1/RM/53 February 2012 -

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