Biological test method for toxicity tests using early life stages of rainbow trout: contents

Table of Contents

• Front Matter
  • Readers' Comments
  • Review Notice
  • Abstract
  • Foreword
  • List of Abbreviations and Chemical Formulae
  • Terminology
  • Acknowledgements
• Section 1: Introduction
  • 1.1 Background
  • 1.2 Historical Use of Test
  • 1.3 Salmonid Species Studied and Recommended
• Section 2: Test Organism
  • 2.1 Species and Life Stages
  • 2.2 Source
• Section 3: Test System
  • 3.1 Facilities and Materials
  • 3.2 Lighting
  • 3.3 Test Apparatus
  • 3.4 Control/Dilution Water
• Section 4: Universal Test Procedures
  • 4.1 Preparing Test Solutions
  • 4.2 Beginning the Test
  • 4.3 Test Conditions and Procedures
    • 4.3.1 Test Options
    • 4.3.2 Test Type and Solution Replacement
    • 4.3.3 Temperature
    • 4.3.4 Dissolved Oxygen and Aeration
    • 4.3.5 pH
    • 4.3.6 Life-stage Transition
    • 4.3.7 Fertilization Success and Thinning
    • 4.3.8 Final Phase of EAF Test
    • 4.3.9 Reference Toxicant
  • 4.4 Test Observations and Measurements
  • 4.5 Test Endpoints and Calculations
    • 4.5.1 Biological Endpoints
    • 4.5.2 Effective and Lethal Concentrations
    • 4.5.3 Inhibiting Concentration for a Specified Percent Effect
    • 4.5.4 NOEC and LOEC
    • 4.5.5 Student's t-test
    • 4.5.6 Tukey's Test
  • 4.6 Test Validity
  • 4.7 Legal Considerations
• Section 5: Specific Procedures for Testing Chemicals
  • 5.1 Test Options
  • 5.2 Properties, Labelling, and Storage of Sample
  • 5.3 Preparing and Aerating Test Solutions
  • 5.4 Control/Dilution Water
  • 5.5 Test Observations and Measurements
  • 5.6 Test Endpoints and Calculations
• Section 6: Specific Procedures for Testing Samples of Effluent, Elutriate, and Leachate
  • 6.1 Test Options
  • 6.2 Sample Collection, Labelling, Transport, and Storage
  • 6.3 Preparing and Aerating Test Solutions
  • 6.4 Control/Dilution Water
  • 6.5 Test Observations and Measurements
  • 6.6 Test Endpoints and Calculations
• Section 7: Specific Procedures for Testing Receiving Water Samples
  • 7.1 Test Options
  • 7.2 Sample Collection, Labelling, Transport, and Storage
  • 7.3 Preparing and Aerating Test Solutions
  • 7.4 Control/Dilution Water
  • 7.5 Test Observations and Measurements
  • 7.6 Test Endpoints and Calculations
• Section 8: Reporting Requirements
  • 8.1 Minimum Requirements for a Test-specific Report
    • 8.1.1 Test Substance
    • 8.1.2 Test Organism
    • 8.1.3 Test Facilities and Apparatus
    • 8.1.4 Control/Dilution Water
    • 8.1.5 Test Method
    • 8.1.6 Test Conditions and Procedures
    • 8.1.7 Test Results
  • 8.2 Additional Reporting Requirements
    • 8.2.1 Test Substance
    • 8.2.2 Test Organism
    • 8.2.3 Test Facilities and Apparatus
    • 8.2.4 Control/Dilution Water
    • 8.2.5 Test Method
    • 8.2.6 Test Conditions and Procedures
    • 8.2.7 Test Results
• References
• Appendices
  1. Members of the Inter-Governmental Aquatic Toxicity Group (as of October, 1998)
  2. Environment Canada, Environmental Protection Service, Regional and Headquarters Offices
  3. Review of Procedural Variations for Undertaking Early Life-stage Tests Using Salmonid Fish
  4. Distribution, Life History, and Husbandry of Rainbow Trout
  5. Logarithmic Series of Concentrations Suitable for Toxicity Tests

List of Tables

1. Recommended Quality for Control/Dilution Water
2. Checklist of Recommended Test Conditions and Procedures

List of Figures

1. Considerations for Preparing and Performing Toxicity Tests Using Early Life Stages of Rainbow Trout and Various Types of Substances
2. General Appearance of Salmonid Early Life Stages
3. Recommended Design for Static-renewal or Flow-through Setups for Incubating Embryos or Alevins in Test Solutions