Biological test method for determining toxicity of sediment using luminescent bacteria: chapter 1

Table of Contents

• Front Matter
  • Readers' Comments
  • Review Notice
  • Abstract
  • Foreword
  • List of Abbreviations and Chemical Formulae
  • Terminology
  • Acknowledgements
• Section 1: Introduction
• Section 2: Test Organisms
  • 2.1 Species
  • 2.2 Source and Holding
• Section 3: Facilities, Equipment, and Supplies
  • 3.1 Facilities
  • 3.2 Apparatus
    • 3.2.1 Cleaning Procedure
    • 3.2.2 Outline of Test and Associated Apparatus
    • 3.2.3 Equipment
    • 3.2.4 Supplies
• Section 4: Procedure for Testing Sediment
  • 4.1 Sample Collection
  • 4.2 Sample Labelling, Transport, and Storage
  • 4.3 Sample Manipulation and Characterization
  • 4.4 Negative and Positive Control Sediment
  • 4.5 Test Conditions
    • 4.5.1 Outline of Test
    • 4.5.2 Manipulations, Adjustments, and Corrections
    • 4.5.3 Temperature
    • 4.5.4 Timing of Events
    • 4.5.5 Test Array
  • 4.6 Test Procedures
    • 4.6.1 Photometer, Water Bath, and Bench Sheet
    • 4.6.2 Subsamples for Moisture
    • 4.6.3 Primary Dilution
    • 4.6.4 Test Concentrations
    • 4.6.5 Reconstitution of Bacterial Reagent
    • 4.6.6 Inoculation and Incubation
    • 4.6.7 Preparing the Computer
    • 4.6.8 Filtration
  • 4.7 Test Measurements and Observations
  • 4.8 Criterion for a Valid Test
• Section 5: Procedure for Testing a Reference Toxicant
• Section 6: Data Analysis and Interpretation
  • 6.1 Data Analysis
  • 6.2 Interpretation of Results
• Section 7: Reporting Requirements
  • 7.1 Minimum Requirements for a Test-Specific Report
    • 7.1.1 Test Material
    • 7.1.2 Test Organisms
    • 7.1.3 Test Facilities and Apparatus
    • 7.1.4 Reconstitution Solution and Solid-Phase Diluent
    • 7.1.5 Test Method
    • 7.1.6 Test Conditions and Procedures
    • 7.1.7 Test Results
  • 7.2 Additional Reporting Requirements
    • 7.2.1 Test Material
    • 7.2.2 Test Organisms
    • 7.2.3 Test Facilities and Apparatus
    • 7.2.4 Reconstitution Solution and Solid-Phase Diluent
    • 7.2.5 Test Method
    • 7.2.6 Test Conditions and Procedures
    • 7.2.7 Test Results
• References
• Appendices
  1. Biological Test Methods and Supporting Guidance Documents Published by Environment Canada’s Method Development & Applications Section
  2. Members of the Inter-Governmental Environmental Toxicity Group (as of April 2002)
  3. Environment Canada Regional and Headquarters Offices
  4. Members of the Scientific Advisory Group
  5. Procedural Variations for Solid-Phase Sediment Toxicity Tests Using Luminescent Bacteria, as Described in Canadian, United States, and Netherlands Methodology Documents
  6. Bench Sheet for Sediment Toxicity Tests Using V. fischeri

List of Tables

1. Checklist of Required or Recommended Test Conditions