Pathogen Safety Data Sheets: Infectious Substances – Ancylostoma duodenale
PATHOGEN SAFETY DATA SHEET - INFECTIOUS SUBSTANCES
SECTION I - INFECTIOUS AGENT
NAME: Ancylostoma duodenale
SYNONYM OR CROSS REFERENCE: Hookworm; Old World HookwormFootnote 1Footnote 2, intestinal nematodeFootnote 1Footnote 2, ground itch Footnote 1Footnote 2, pruritic erythematous rashFootnote 1Footnote 2, Ankylostomiasis, anchylostomiasis, ancylostomiasis, helminthiasisFootnote 3, miners' anemiaFootnote 4, tunnel diseaseFootnote 4, uncinariasisFootnote 4, brickmaker's anemiaFootnote 5.
CHARACTERISTICS: A. duodenale belongs to the family Ancylostomatidae and subfamily of Ancylostomatinae Footnote 1. Adults are slightly larger and more robust than N. americanus. Adult male worm measures 8 to 11 by 0.4-0.5 mm, while the female is 10 to 13 mm long by 0.4-0.5 mm wide Footnote 1, Footnote 2. Their heads continue in the same direction as the curvature of their bodies Footnote 2, Footnote 6. Eggs are oval shaped with a thin shell and measure approximately 56-75 μm by 36-40 μm. They are characterized by a clear space between the developing embryo and the egg shell Footnote 1, Footnote 2. Eggs of N. americanus and A. duodenale are indistinguishable. Eggs are passed in feces and then embryonate and hatch within 1-2 days in soil as first stage rhabditoid larvae. First stage larvae develop to third stage, infectious, filariform larvae in approximately 1 week Footnote 1, Footnote 2, Footnote 7, Footnote 8.
SECTION II - HAZARD IDENTIFICATION
PATHOGENICITY/TOXICITY: Like N. americanus, A. duodenale (third-stage) larvae follow thermal gradients and are guided by heat to human skin where they may irritate or invade the skinFootnote 9. Like N. americanus, a pruritic, erythematous, papular rash develops and produces what is known as “ground itch” around penetration sites of the infective larvae, usually on the hands and feetFootnote 1Footnote 2. Invasive infection occurs when larvae enter the bloodstream and are carried to the lungs. A mild cough and pharyngeal irritation may occur during larval migration in the airways; however, passage through the lungs is usually asymptomaticFootnote 10. The larvae are swallowed down the oesophagus and migrate to the gastrointestinal tract. Larvae then hook onto the intestinal mucosa where they mature into adult hookworms by feeding on blood. The major-hookworm related injury occurs due to intestinal blood loss, which can lead to iron-deficiency anaemia in moderate to heavy infections. In the gut, adult worms may produce epigastric pain and abnormal peristalsisFootnote 2Footnote 6. Clinical features are highly dependent on worm burden and dietary iron levelsFootnote 1Footnote 2Footnote 6. In children this condition may precipitate heart failure or kwashiorkorFootnote 1. Retardation of mental, sexual, and physical development has also been noted in childrenFootnote 1Footnote 2Footnote 7. Intrauterine growth retardation, prematurity, and low birth weight among newborns born to infected mothers has been notedFootnote 1Footnote 7. Acute/heavy infections may result in fatigue, weakness, abdominal pain, and diarrhea with blood lossFootnote 1. Adult’s lifespan in human intestine is 1-2 years but can be as long as 10-12 yearsFootnote 1Footnote 2Footnote 6Footnote 11.
EPIDEMIOLOGY: Infections with A. duodenale frequently overlap N. americanus infections in areas of Africa, India and China. Estimated that approximately 740 million individuals are currently infected worldwide with A. duodenale and N. americanus Footnote 1, Footnote 2, Footnote 12, Footnote 13.
Mortality rate, worldwide, attributed to deaths occurring through direct hookworm infection, is estimated to be ~65,000 deaths annually Footnote 14. The majority of infected individuals have a small worm burden and are asymptomatic Footnote 1, Footnote 2. Hookworm infection is among the most important tropical diseases in humans Footnote 7. Hookworm infection is found worldwide between 45°N and 30°S latitudes. A. duodenale is seen in Mediterranean basin, Middle East, sub-Saharan Africa, northern India, China, and Japan Footnote 1, Footnote 2, Footnote 6, Footnote 7. A. duodenale is endemic in warm, moist tropical areas where people defecate in the soil Footnote 6. Infection levels intensify in closed, densely populated communities such as tea and coffee plantations Footnote 2.
HOST RANGE:Humans Footnote 15.
INFECTIOUS DOSE: Unknown.
MODE OF TRANSMISSION: Direct contact of soil containing filariform larvae with unprotected skin Footnote 1, Footnote 2, Footnote 6, Footnote 7. Infection can also be oral with direct maturation in the intestine to adult stage Footnote 1, Footnote 7. Infection can be transplacental or transmammary from mother to fetus/infant via infected placental/mammary tissue Footnote 16-Footnote 18.
INCUBATION PERIOD: Following penetration, A. duodenale migrates to the lungs within about 10 days Footnote 19. After 3 to 5 weeks, it passes through the gastrointestinal tract and attaches to the intestinal mucosa, where it matures into an adult worm and may stay for up to 1 year. Patent infections develop in 5-8 weeks following exposure Footnote 1, Footnote 2, Footnote 6. However, A. duodenale have the ability to arrest development in the host, this phenomenon can extend the prepatent period Footnote 20.
SECTION III - DISSEMINATION
RESERVOIR: Humans are the only known reservoir of A.duodenale and N. americanus. The majority of infected individuals are asymptomatic due to low worm burden; however, eggs can be passed in fecesFootnote 1Footnote 2Footnote 6Footnote 7.
ZOONOSIS: None. However, transfer of adult A. duodenale from infected dogs to a 3 year old child afflicted with congenital polycythemia has been reported Footnote 21.
SECTION IV - STABILITY AND VIABILITY
SUSCEPTIBILITY TO DISINFECTANTS: Infective larvae of A. duodenale are susceptible to 70% ethanol for 10 minutes, 0.5 % Dettol® for 20 minutes, chlorinated hydrocarbons (tetrachloroethylene) Footnote 22. Sodium hypochlorite (1%) and glutaraldehyde (2%) are not effective Footnote 22, Footnote 23.
PHYSICAL INACTIVATION: Infective larvae are inactivated by the application of heat through water above 80°C (practical recommendation is to use water that is close to boiling point) Footnote 22. Ancylostoma spp. larvae can not survive drying, or direct sunlight, and they cannot survive below 0 ºC or above 45 ºC Footnote 24. The larvae are also sensitive to high salt concentrations Footnote 25.
SURVIVAL OUTSIDE HOST: Eggs hatch, releasing first stage rhabditiform larvae in soil, which develop into infectious, filariform larvae in 5-8 days Footnote 1, Footnote 2, Footnote 6, Footnote 7. Infectious, filariform larvae do not feed and if they fail to infect a host they die within a few weeks Footnote 2, Footnote 6. The infective filariform larvae remain viable in the soil for several weeks Footnote 1.
SECTION V – FIRST AID / MEDICAL
SURVEILLANCE: Monitor for symptoms. Infection is demonstrated by direct observation of characteristic eggs in feces Footnote 1Footnote 7. Quantitative egg counts include Kato-Katz test, concentration test, Beaver direct egg count, Stoll dilutional egg count, and McMaster techniques Footnote 26; and PCR-based tests, with PCR-based tests being the most sensitive Footnote 26.
FIRST AID/TREATMENT: Widely used antihelmintic agents pyrantel pamoate, mebendazole, and albendazole are highly effective in treating A. duodenale infection Footnote 1, Footnote 2, Footnote 6, Footnote 7. When anaemia is mild or moderate, iron replacement is often adequate treatment Footnote 1, Footnote 2, Footnote 6. More severe anaemia may require blood transfusions Footnote 1, Footnote 2.
PROPHYLAXIS: Hygiene measures such as hand washing, drinking safe water, properly cleaning and cooking food, and wearing shoes Footnote 10.
SECTION VI - LABORATORY HAZARDS
LABORATORY-ACQUIRED INFECTIONS: None reported for A. duodenale. One case of ground itch reported in an animal caretaker treating cat infected with Ancylostoma braziliense and Ancylostoma caninum – Close relatives of A. duodenale Footnote 28, Footnote 29.
SPECIAL HAZARDS: None.
SECTION VII – EXPOSURE CONTROLS / PERSONAL PROTECTION
RISK GROUP CLASSIFICATION: Risk Group 2Footnote 30.
CONTAINMENT REQUIREMENTS: Containment Level 2 facilities, equipment, and operational practices for work involving infectious or potentially infectious materials, animals, or cultures Footnote 31.
PROTECTIVE CLOTHING: Lab coat. Gloves when direct skin contact with infected materials or animals is unavoidable. Eye protection must be used where there is a known or potential risk of exposure to splashes Footnote 31.
OTHER PRECAUTIONS: All procedures that may produce aerosols, or involve high concentrations or large volumes should be conducted in a biological safety cabinet (BSC). The use of needles, syringes, and other sharp objects should be strictly limited. Additional precautions should be considered with work involving animals or large scale activities Footnote 31.
SECTION VIII – HANDLING AND STORAGE
SPILLS: Allow aerosols to settle and, wearing protective clothing, gently cover spill with paper towels and apply an appropriate disinfectant, starting at the perimeter and working towards the centre. Allow sufficient contact time before clean up Footnote 31, Footnote 32.
DISPOSAL: Decontaminate all wastes that contain or have come in contact with the infectious organism by autoclave, chemical disinfection, gamma irradiation, or incineration before disposing Footnote 31.
STORAGE: The infectious agent should be stored in leak-proof containers that are appropriately labelled Footnote 31.
SECTION IX - REGULATORY AND OTHER INFORMATION
UPDATED: December 2011
PREPARED BY: Pathogen Regulation Directorate, Public Health Agency of Canada.
Although the information, opinions and recommendations contained in this Pathogen Safety Data Sheet are compiled from sources believed to be reliable, we accept no responsibility for the accuracy, sufficiency, or reliability or for any loss or injury resulting from the use of the information. Newly discovered hazards are frequent and this information may not be completely up to date.
Public Health Agency of Canada, 2011
- Footnote 1
Sheorey, H., Biggs, B. A., & Traynor, P. (2007). Nematodes. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry & M. A. Pfaller (Eds.), Manual of Clinical Microbiology (9th ed., pp. 2144-2155.). Washington, D.C.: ASM press.
- Footnote 2
Plourde, J. J. (2004). Intestinal Nematodes. In K. J. Ryan, & C. G. Ray (Eds.), Sherris Medical Microbiology: An Introduction to Infectious Disease (4th ed., pp. 763-777). USA: McGraw-Hill.
- Footnote 3
Loughlin, E. H., & Spitz, S. H. (1949). Diagnosis of Helminthiasis. JAMA: The Journal of the American Medical Association, 139(15), 997-1000.
- Footnote 4
A Factor in the Anemia of Uncinariasis. (1904). JAMA: The Journal of the American Medical Association, XLIII(12), 817-b.
- Footnote 5
Bozzolo, C. (1912). Notes on the Treatment of Ankylostoma Anemia (uncinariasis, hookworm disease) with Thymol. JAMA: The Journal of the American Medical Association, LVIII(23), 1744-1746.
- Footnote 6
Cross, J. H. (1996). Enteric Nematodes of Humans in Medical Microbiology. In Baron S. et al. (Ed.), (4th Edition ed., ). Galveston: The University of Texas Medical Branch.
- Footnote 7
Hotez, P. J., Brooker, S., Bethony, J. M., Bottazzi, M. E., Loukas, A., & Xiao, S. (2004). Hookworm infection. New England Journal of Medicine, 351(8), 799-807.
- Footnote 8
Collins, C. H., & Kennedy, D. A. (1999). Endoparasites. Laboratory-acquired Infections: History, incidence, causes, and prevention (4th ed., pp. 253-255). Oxford: Butterworth Heimann.
- Footnote 9
Haas, W., Haberl, B., Syafruddin, Idris, I., Kallert, D., Kersten, S., Stiegeler, P., & Syafruddin. (2005). Behavioural strategies used by the hookworms Necator americanus and Ancylostoma duodenale to find, recognize and invade the human host. Parasitology Research, 95(1), 30-39. doi:10.1007/s00436-004-1257-7
- Footnote 10
Weller, P. R., & Leder, K.Hookworm infection. www.uptodate.com
- Footnote 11
Brooker, S., Bethony, J., & Hotez, P. J. (2004). Human hookworm infection in the 21st century. Advances in Parasitology, 58, 197-288.
- Footnote 12
Hotez, P. J., Le Trang, N., & Cerami, A. (1987). Hookworm antigens: the potential for vaccination. Parasitology Today, 3(8), 247-249.
- Footnote 13
Hotez, P. J., Zhan, B., Bethony, J. M., Loukas, A., Williamson, A., Goud, G. N., Hawdon, J. M., Dobardzic, A., Dobardzic, R., Ghosh, K., Bottazzi, M. E., Mendez, S., Zook, B., Wang, Y., Liu, S., Essiet-Gibson, I., Chung-Debose, S., Xiao, S., Knox, D., Meagher, M., Inan, M., Correa-Oliveira, R., Vilk, P., Shepherd, H. R., Brandt, W., & Russell, P. K. (2003). Progress in the development of a recombinant vaccine for human hookworm disease: the Human Hookworm Vaccine Initiative. International Journal for Parasitology, 33(11), 1245-1258.
- Footnote 14
Diemert, D. J., Bethony, J. M., & Hotez, P. J. (2008). Hookworm vaccines. Clinical Infectious Diseases, 46(2), 282-288.
- Footnote 15
American Academy of Pediatrics.Committee on Infectious Diseases. (2009). Red book (28th ed.). Elk Grove Village, IL: American Academy of Pediatrics. Retrieved from http://online.statref.com/document.aspx?FxId=76&DocID=1&grpalias=
- Footnote 16
Hotez, P. J. (1989). Hookworm disease in children. Pediatric Infectious Disease Journal, 8(8), 516-520.
- Footnote 17
Yu, S. H., Jiang, Z. X., & Xu, L. Q. (1995). Infantile hookworm disease in China. A review. Acta Tropica, 59(4), 265-270.
- Footnote 18
Brooker, S., Hotez, P. J., & Bundy, D. A. (2008). Hookworm-related anaemia among pregnant women: a systematic review. PLoS Neglected Tropical Diseases [Electronic Resource], 2(9), e291.
- Footnote 19
Sheorey, H., Biggs, B. A., & Traynor, P. (2007). Nematodes. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry & M. A. Pfaller (Eds.), Manual of Clinical Microbiology (9th ed., pp. 2144-2155). Washington, USA: ASM Press.
- Footnote 20
Schad, G. A., Murrell, K. D., Fayer, R., El Naggar, H. M. S., Page, M. R., Parrish, P. K., & Stewart, T. B. (1984). Paratenesis in Ancylostoma duodenale suggests possible meat-borne human infection. Transactions of the Royal Society of Tropical Medicine and Hygiene, 78(2), 203-204.
- Footnote 21
Walterspiel, J. N., Schad, G. A., & Buchanan, G. R. (1984). Direct transfer of adult hookworms (Ancylostoma duodenale) from dog to child for therapeutic purposes. Journal of Parasitology, 70(2), 217-219.
- Footnote 22
Speare, R., Melrose, W., Cooke, S., & Croese, J. (2008). Techniques to kill infective larvae of human hookworm Necator americanus in the laboratory and a new Material Safety Data Sheet. Australian Journal of Medical Science, 29(3), 91-96.
- Footnote 23
Ayçİçek, H., Yarsan, E., Oğuz Sarimehmetoğlu, H., Tanyüksel, M., Gİrgİnkardeşler, N., & Özyurt, M. (2001). Efficacy of Some Disinfectants on Embryonated Eggs of Toxocara canis. Turk J Med Sci, 31(1), 35-39.
- Footnote 24
Menelaos, L. A., & Smaragda, K. E. (2006). Prevalence of hookworm parasites in dog from the area of Thessaloniki and their zoonotic importance. Buletin USAMV-CN, 63, 297-303.
- Footnote 25
Pawlowski, Z. S., Schad, G. A., & Stott, G. J. (1991). Epidemiology of hookworm infection. Hookworm infection and anaemia: Approaches to prevention and control (). Geneva: World Health Organization.
- Footnote 26
Pawlowski Z.S., Schad G.A., Stott G.J. (1991). Hookworm infection and anaemia : approaches to prevention and control. Geneva: World Health Organization.
- Footnote 27
Hawdon, J. M., & Hotez, P. J. (1996). Hookworm: developmental biology of the infectious process. Current Opinion in Genetics & Development, 6(5), 618-623.
- Footnote 28
Herwaldt, B. L. (2001). Laboratory-acquired parasitic infections from accidental exposures. Clinical Microbiology Reviews, 14(4), 659-688.
- Footnote 29
Stone, O. J., & Levy, A. (1967). Creeping eruption in an animal caretaker. Laboratory Animal Care, 17(5), 479-482.
- Footnote 30
Human Pathogens and Toxins Act. S.C. 2009, c. 24. Government of Canada, Second Session, Fortieth Parliament, 57-58 Elizabeth II, 2009, (2009).
- Footnote 31
Public Health Agency of Canada. (2004). In Best M., Graham M. L., Leitner R., Ouellette M. and Ugwu K. (Eds.), Laboratory Biosafety Guidelines (3rd ed.). Canada: Public Health Agency of Canada.
- Footnote 32
Burnett, L. A. C., Lunn, G., & Coico, R. (2009). Biosafety: Guidelines for working with pathogenic and infectious microorganisms. Current Protocols in Microbiology, (SUPPL. 13), 1A.1.1-1A.1.14.
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