Pathogen Safety Data Sheets: Infectious Substances – Vibrio cholerae
PATHOGEN SAFETY DATA SHEET - INFECTIOUS SUBSTANCES
SECTION I - INFECTIOUS AGENT
NAME: Vibrio cholerae, serogroup O1, serogroup O139 (Bengal)
SYNONYM OR CROSS REFERENCE: Cholera
CHARACTERISTICS: Vibrio cholerae is a gram negative, non-spore forming, curved rod that is oxidase positiveFootnote 1Footnote 2Footnote 3. It is very motile and has a single polar flagellumFootnote 1. The bacterium is 1- 3 µm by 0.5-0.8 µm, is a facultative anaerobe and is part of the Vibronaceae familyFootnote 1Footnote 3. Serogroups O1 (classical and El Tor biotypes) and O139 are primarily responsible for cholera outbreaksFootnote 1Footnote 3. Pathogenic serogroups produce cholera toxin (CT) while non pathogenic strains may or may not produce this toxinFootnote 2. Recently, V. cholerae serougroup O75 strains possessing the cholera toxin gene were isolated from patients with severe diarrhoea, and serogroup O141 has been associated with sporadic cholera-like diarrhoea and bloodstream infections in the United StatesFootnote 4Footnote 5. Some serotypes may serve as a reservoir for the cholera toxin phage genomeFootnote 6Footnote 7. Serotypes that do not produce cholera toxin can still cause illness in humans (i.e. enteritis)Footnote 8.
SECTION II - HAZARD IDENTIFICATION
PATHOGENICITY/TOXICITY: Vibrio cholerae can cause syndromes ranging from asymptomatic to cholera gravisFootnote 3. In endemic areas, 75% of cases are asymptomatic, 20% are mild to moderate, and 2-5% are severe forms like cholera gravisFootnote 3. Symptoms include abrupt onset of watery diarrhoea (a grey and cloudy liquid), occasional vomiting and abdominal crampsFootnote 1Footnote 3. Dehydration ensues with symptoms and signs such as thirst, dry mucous membranes, decreased skin turgor, sunken eyes, hypotension, weak or absent radial pulse, tachycardia, tachypnea, hoarse voice, oliguria, cramps, renal failure, seizures, somnolence, coma and deathFootnote 1. Death due to dehydration can occur in a hours to days in untreated children and the disease is dangerous for pregnant women and their foetuses during late pregnancy as abortion, premature labor and fetal death may occurFootnote 3Footnote 9Footnote 10. In cases of cholera gravis involving severe dehydration, up to 60% of patients can die; however, less than 1% of cases treated with rehydration therapy are fatal. The disease typically lasts from 4-6 daysFootnote 3Footnote 11. Worldwide, diarrhoeal disease, caused by cholera and many other pathogens, is the second leading cause of death for children under the age of 5 and at least 120,000 deaths are estimated to be caused by cholera each yearFootnote 12Footnote 13. In 2002, the WHO deemed that the case fatality ratio for cholera was about 3.95%Footnote 3.
EPIDEMIOLOGY: In the past 200 years, there have been 8 main cholera pandemics, with the disease being most common in tropical and subtropical areasFootnote 2Footnote 14. Most of the cases are in the Indian subcontinent and Africa (in 2002 the WHO estimated that 97% of the cholera cases were in Africa)Footnote 2Footnote 3. There are several million cases of cholera each year and in endemic areas, these tend to be most common in children aged 2-9 and in women who are of child-bearing ageFootnote 3Footnote 12. Epidemics in endemic areas tend to occur during the hot seasonFootnote 1.
HOST RANGE: Humans, water birds, shellfish, fish, and herbivores have been found to contain the infectious agentFootnote 1.
INFECTIOUS DOSE: The infectious dose ranges between 10Footnote 6 and 10Footnote 11 ingested vibriosFootnote 1. The infectious dose depends on gastric acidity (lower acidity levels reduces the number of vibrios required for infection)Footnote 1.
MODE OF TRANSMISSION: Cholera is typically spread by consumption of water that is contaminated with infectious fecesFootnote 1Footnote 2. Epidemics caused by infectious raw fish and seafood have been reportedFootnote 1.
INCUBATION PERIOD: The incubation period can range from a few hours to 5 days after infectionFootnote 1.
COMMUNICABILITY: Symptomatic patients may shed vibrios before clinical signs of illness and up to 2 weeks after, whereas asymptomatic patients typically only shed vibrios for 1 dayFootnote 12. A carrier state (where the patient has the infectious agent without any clinical manifestations) can exist for several weeks where vibrios are shed in small and intermittent quantities (15), (16).
SECTION III - DISSEMINATION
RESERVOIR: Humans are a reservoir for the disease as are animals around aquatic environmentsFootnote 1. The bacterium has been found in birds and herbivores surrounding freshwater lakes and rivers as well as in algae, copepods (zooplankton), crustaceans and insectsFootnote 1Footnote 3.
SECTION IV - STABILITY AND VIABILITY
DRUG SUSCEPTIBILITY: Susceptible to antibiotics. Tetracycline has been the drug of choice, although resistance to this antibiotic is becoming more commonFootnote 17Footnote 18. Ciproflaxin, doxycycline and co-trimoxazole can also be usedFootnote 1. An outbreak in 1979 in Bangladesh was caused by multi-drug resistant strains of El Tor biotypeFootnote 18. 36% of strains in this outbreak were resistant to tetracycline, ampicillin, kanamycine, streptomycin, and trimethoprim sulfamethoxazoleFootnote 18.
DRUG RESISTANCE: Resistance has been shown to nalidixic acid, furazolidone, and co- trimoxazole, V. cholerae O1 lnaba isolates have been found to be muli-antibiotic resistant, when increasing resistance to ciprofloxacinFootnote 19.
SUSCEPTIBILITY TO DISINFECTANTS: Susceptible to 2-5% phenol, 1% sodium hypochlorite, 4% formaldehyde, 2% glutaraldehyde, 70% ethanol, 70% propanol, 2% peracetic acid, 3-6% hydrogen peroxide, and 0.16% iodineFootnote 14.
PHYSICAL INACTIVATION: Vibrio cholerae is sensitive to cold (loss of viability after a cold shock at 0ºC)Footnote 20.
SURVIVAL OUTSIDE HOST: Cholera can survive in well water for 7.5 ± 1.9 days and the El Tor biotype can survive 19.3 ± 5.1 daysFootnote 21. The bacterium can survive in a wide variety of foods and drinks for 1-14 days at room temperature and 1-35 days in an ice boxFootnote 21. It has also been found on fomites at room temperature for 1-7 daysFootnote 21.
SECTION V - FIRST AID / MEDICAL
Note: All diagnostic methods are not necessarily available in all countries.
FIRST AID/TREATMENT: Fluid replacement, electrolyte replacement and base i.v. fluid replacement followed by the WHO's oral rehydration solution (Na+ 90 mmol/L, K+ 20 mmol/L, Cl- 80 mmol/L, citrate (10 mmol/L and glucose 110 mmol/L) is the recommended treatment for dehydrationFootnote 1. Administering an antibiotic like ciproflaxin, doxycycline or co-trimoxazole reduces the duration of the illnessFootnote 1.
IMMUNIZATION: Routine vaccination for laboratory workers and travellers is not recommendedFootnote 22Footnote 23. Traditional parenteral inactivated vaccine strains are available though not recommended for widespread use as they only provide protection for 3-6 monthsFootnote 3Footnote 14. Oral vaccines that provide protection for several years (up to 3) are available but their efficacy in endemic areas has not been confirmedFootnote 3Footnote 13.
PROPHYLAXIS: Chemoprophylaxis with antibiotics has not been shown to be effectiveFootnote 1. Proper hygiene, sanitary measures, water treatment and careful food preparation are the best prophylactic measures in endemic areasFootnote 1.
SECTION VI - LABORATORY HAZARDS
LABORATORY-ACQUIRED INFECTIONS: 12 cases of infection with 4 deaths were reported up to 1979Footnote 24. The deaths were associated with mouth pipetting, contact with infectious feces and contaminated laboratory laundryFootnote 24.
SOURCES/SPECIMENS: Feces and naturally or experimentally infected animals are the main specimens which contain the infectious agentFootnote 22.
PRIMARY HAZARDS: The primary hazards when working with this agent are ingestion and accidental parenteral inoculationFootnote 9Footnote 14Footnote 22. The risk of aerosol exposure is not knownFootnote 22.
SPECIAL HAZARDS: The risk of infection is higher in people who don't have gastric acid (i.e. due to gastrectomy or achlorhydria)Footnote 2.
SECTION VII - EXPOSURE CONTROLS / PERSONAL PROTECTION
RISK GROUP CLASSIFICATION: Risk group 2Footnote 25.
CONTAINMENT REQUIREMENTS: Containment Level 2 facilities, equipment, and operational practices for work involving infectious or potentially infectious materials, animals, or cultures.
PROTECTIVE CLOTHING: Lab coat. Gloves when direct skin contact with infected materials or animals is unavoidable. Eye protection must be used where there is a known or potential risk to splashesFootnote 26.
OTHER PRECAUTIONS: All procedures that may produce aerosols, or involve high concentrations or large volumes should be conducted in a biological safety cabinet (BSC). The use of needles, syringes, and other sharp objects should be strictly limited. Additional precautions should be considered with work involving animals or large scale activitiesFootnote 26.
SECTION VIII - HANDLING AND STORAGE
SPILLS: Allow aerosols to settle and, wearing protective clothing, gently cover spill with paper towels and apply appropriate disinfectant, starting at the perimeter and working towards the centre. Allow sufficient contact time before clean up (30 min)Footnote 26.
DISPOSAL: All wastes should be decontaminated before disposal either by steam sterilization, incineration or chemical disinfectionFootnote 26.
STORAGE: The infectious agent should be stored in a sealed and identified containerFootnote 26.
SECTION IX - REGULATORY AND OTHER INFORMATION
REGULATORY INFORMATION: The import, transport, and use of pathogens in Canada is regulated under many regulatory bodies, including the Public Health Agency of Canada, Health Canada, Canadian Food Inspection Agency, Environment Canada, and Transport Canada. Users are responsible for ensuring they are compliant with all relevant acts, regulations, guidelines, and standards.
UPDATED: September 2010
PREPARED BY: Pathogen Regulation Directorate, Public Health Agency of Canada.
Although the information, opinions and recommendations contained in this Pathogen Safety Data Sheet are compiled from sources believed to be reliable, we accept no responsibility for the accuracy, sufficiency, or reliability or for any loss or injury resulting from the use of the information. Newly discovered hazards are frequent and this information may not be completely up to date.
Public Health Agency of Canada, 2010
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