Official Methods for the Microbiological Analysis of Foods

The documents in Volume 1 of the Compendium of Analytical Methods are available upon request.

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Table of Contents - Volume 1 - The Compendium of Analytical Methods
Title Date Issued
Preface Preface to the Compendium of Analytical Methods January 2003
Disclaimer Disclaimer for the Compendium of Analytical Methods January 2005
Terms of Reference Terms of Reference for the Microbiological Methods Committee June 2010
Interpretive Summary Health Products and Food Branch (HPFB) Standards and Guidelines for Microbiological Safety of Food - An Interpretive Summary April 2008
Extraneous Materials Overview Guidelines for the General Cleanliness of Food - An Overview March 2009
Development of Methods Guidelines for Modifying or Revoking Food Microbiological Methods July 2012
Template for MMC Consultation on Qualitative Relative Validation Study Design November 2017
Part 1. Definitions and Explanations of Methods and General Procedures April 2008
Part 2A: Data Requirements And Submission Templates For Methods and Quantitative Methods May 2008
Part 2B: Data Requirements and Submission Templates for Qualitative Methods February 2019

Part 3: Guidelines for the Evaluation of Quantitative Food Microbiological Methods

February 2015
February 2015
February 2015
Part 4: Guidelines for the Relative Validation of Indirect Qualitative Food Microbiological Methods March 2011
March 2011
May 2011
March 2015
Part 5: Guidelines to Verify Standard Food Microbiological Methods for Implementation in Routine Testing April 2015
Part 6: Guidelines for the Relative Validation of Qualitative Microbiological Methods for Testing Environmental Surface Samples from Food Production Environments for Pathogenic Microorganisms July 2017
Part 6B: Data Requirements and Submission Templates for Qualitative Environmental Surface Methods February 2019
Part 7: Validation of Platform Upgrades for Rapid Test Methods December 2011

Part 9: Guidelines for the Validation of Colony Identification Methods

July 2016
Guidelines PCR Technology BMH Guidelines on the Use of PCR Technology for the Detection of Bacterial Foodborne Pathogens

September 2012

MFO-2 Microbiological Examination of Ice Cream and Ice Milk November 1981
MFO-3 Determination of Phosphatase Activity in Dairy Products November 1981
MFO-4 Microbiological Examination of Cottage Cheese November 1981
MFO-5 Examination of Canned Tomatoes, Tomato Juice and Vegetable Juice, Tomato Puree, Tomato Paste, Tomato Pulp and Tomato Catsup for Mould Filaments November 1981
MFO-6 Microbiological Examination of Egg Products and of Liquid Eggs November 1981
Supplement to All Official Methods (MFO-6, MFO-10, MFO-11 and MFO-12) That Detect Salmonella March 2009
MFO-7 Microbiological Examination of Milk November 1981
MFO-8 Determination of Sediments in Milk November 1981
MFO-9 Microbiological Examination of Mineral Water November 1981
MFO-10 Microbiological Examination of FrogLegs November 1981
Supplement to All Official Methods (MFO-6, MFO-10, MFO-11 and MFO-12) That Detect Salmonella March 2009
MFO-11 Microbiological Examination of Cocoa and Chocolate November 1981
Supplement to All Official Methods (MFO-6, MFO-10, MFO-11 and MFO-12) That Detect Salmonella March 2009
MFO-12 Microbiological Examination of Milk Powder November 1981
Supplement to All Official Methods (MFO-6, MFO-10, MFO-11 and MFO-12) That Detect Salmonella March 2009
MFO-14 Microbiological Examination of Cheese November 1983
Note of Interpretation for Method MFO-14 April 2012
MFO-15 Microbiological Examination of Water in Sealed Containers (excluding Mineral and Spring Water) and of Prepackaged Ice November 1981
Appendix A Definitions of Terms November 2006
Appendix B Collection and Transportation of Samples November 2006
Appendix C Calculation of the Most Probable Number of Growth Units for Hydrophobic Grid-Membrane Filter (HGMF) Methods May 2014
Appendix D Calculation of the Most Probable Number (MPN) January 1993
Appendix E Supplement to All Compendium Methods March 2024
Appendix G Glossary of Agars, Broths and Other Reagents  
Index and Introduction March 2021
Section A January 2006
Section B January 2006
Section C January 2006
Section D January 2006
Section E January 2006
Section F January 2006
Section G January 2006
Section H January 2006
Section I January 2006
Sections J-K January 2006
Section L January 2006
Section M January 2006
Section N January 2006
Section O January 2006
Section P February 2006
Sections Q-R January 2006
Section S January 2008
Section T February 2006
Section U January 2006
Section V January 2006
Section W January 2006
Sections X-Y January 2006
Appendix J Supplement to All Methods That Detect Salmonella November 2003
Appendix K Summaries of Methods that Detect Specific Bacteria in this Compendium  
Escherichia coli O157:H7 and other Verotoxigenic E. coli April 2015
Listeria spp. and Listeria monocytogenes June 2013
Staphylococcus aureus or Staphylococcus enterotoxin June 2013
Salmonella April 2016
Appendix L Confirmation Steps for Methods for the Detection of Listeria spp. in Foods and Environmental Samples Removed July 2012
Appendix M Supplement to All Bacterial Qualitative Methods in the Compendium of Analytical Methods: Interpretation of Compositing June 2014

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